Diff-Quick Staining Effectiveness in 24-Hour Stored Diabetes Mellitus Urine

Abstract

Diabetes Mellitus is a chronic metabolic disorder characterized by hyperglycemia due to abnormalities in insulin secretion, insulin action, or both. High blood glucose levels in patients with Diabetes Melitus can cause glucose to be detected in the urine (glucosuria), which reflects impaired renal filtration conditions. The urine of diabetic patients has certain characteristics that may affect laboratory examinations, including cytological examinations. Diff quick staining is one of the cytology staining methods that is derived from Romanowsky staining. This staining is widely used because the process is fast, efficient, and able to provide good color contrast in the nucleus and cytoplasm of cells. However, the quality of the staining results is greatly influenced by the sample conditions, including the time of the examination delay. This study aims to determine the impact of delaying the examination of urine samples for 24 hours on the quality of diff quick coloring in the urine of patients with Diabetes Mellitus. The study used a descriptive design with purposive sampling technique, involving 25 urine samples of type II Diabetes Melitus patients. The results showed that most of the urine samples had poor diff quick staining quality, even on the first day of sampling. A 24-hour storage delay caused a significant decrease in staining quality. This study concludes that delaying the examination of urine samples can affect the staining quality of urine samples.